Visn. Nac. Akad. Nauk Ukr. 2018. (3): 73-78  

Ye.A. Shesterenko
Bogatsky Physico-Chemical Institute of the National Academy of Sciences of Ukraine, Odesa

According to the materials of scientific report at the meeting of the Presidium of NAS of Ukraine, December 13, 2017

Methods of carboxylesterase immobilization as a component of pig liver microsomal fraction immobilization in polyvinyl alcohol, phyllophorine from Phyllophora nervosa and sodium alginate were developed. The highly active biocatalysts (65–80% retaining of initial esterase activity), stable at storage and to elevated temperatures action, were obtained. Biotechnologies of 3-hydroxy-1,4-benzodiazepin-2-one esters enantioselective hydrolysis with a help of free and immobilized microsomal fraction preparation were created. As a result of enzymatic hydrolysis the S-enantiomers of substrates, with increased affinity to central benzodiazepine receptors and anticonvulsant activity, were obtained for the first time. The immobilized pig liver microsomal fraction preparations, as it was shown, catalyzed enantioselective hydrolysis of 3-hydroxy-1,4-benzodiazepin-2-one esters with 50% degree of transformation during 5-12 cycles of usage in batch mode.
Keywords: immobilization, pig liver microsomal fraction, polymeric supports, enantioselective hydrolysis, 3-hydroxy-1,4-benzodiazepin-2-one esters.

Language of article: ukrainian



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