Visn. Nac. Akad. Nauk Ukr. 2019. (10): 38-43
https://doi.org/10.15407/visn2019.10.038

Iu.P. Mukha
Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine, Kyiv

PLASMONIC MONO- AND BIMETALLIC NANOPARTICLES OF GOLD AND SILVER AS PROMISING TOOLS OF COMPLEX THERAPY OF CANCER
According to the materials of scientific report at the meeting of the Presidium of NAS of Ukraine, September 11, 2019

In the presented work, a systematic approach is developed to prepare stable colloids of noble metal nanoparticles (NPs) as promising agents for cancer treatment. Mono- and bimetallic gold and silver NPs of alloy and core-shell type were prepared in aqueous medium using biocompatible component tryptophan as metal reducer and particle stabilizer.

The impact of metal components/composition as well as metal topological distribution within NPs on optical properties and biological activity is discussed. Only one localized surface plasmon resonance (LSPR) band is characteristic for bimetallic NPs. In case of alloy Ag/Au nanoparticles the maximum is located between the maxima inherent to the LSPR bands of individual metals and shifts from 411 nm (silver) to 526 nm (gold) with decreasing Ag:Au molar ratio. In case of bimetallic core-shell NPs LSPR absorption band of previously formed monometallic particles, used as a core, disappears during the synthesis while LSPR band of the second metal grows in the spectra of the same colloid. Among studied alloy systems Ag/Au nanoparticles with metal ratio 3:1 had the maximal anticancer effect towards HCT116, 4T1, HUH7 cell lines, while the toxicity was found significantly decreased in non-cancerous HEK293 cell lines. During antitumor activity studies in vivo on mouse with Lewis lung carcinoma treated with alloy (Ag:Au = 3:1, 1:1, 1:3) and core-shell nanoparticles. (Au core/Ag shell, Ag core/Au shell), the system with Ag core covered by Au shell appeared to be the most effective.

The synthesis of nanoparticles with controlled properties was demonstrated with the use of chemical and photochemical methods. The activation of redox process between metal ions and tryptophan with UV irradiation at 280 nm, corresponding to the position of the indole absorption band of amino acid, allows to affect the optical properties of the system, characteristics of nanoparticles and their self-assembling in colloid depending on experimental parameters like temperature of reaction, output optical power density and duration of irradiation.

Chemical and photochemical methods were applied for preparation of magneto-plasmonic nanostructures composed of magnetic iron oxide core and gold/silver shell. The optical properties of magneto-plasmonic Fe3O4/Au(Ag) nanocomposites, namely position of plasmon resonance band maximum and absorbance in near infrared region, can be adjusted by varying the temperature of reaction from 25 to 100°C and applying UV irradiation. Such nanosystems possess properties inherent to both individual components and can be applied in biomedical field for targeted drug delivery and therapy.
Keywords: nanoparticles, gold, silver, bimetallic, localized surface plasmon resonance, tryptophan, magneto-plasmonic nanosystems.

 Language of article: ukrainian

Full text (PDF)

REFERENCES

  1. Sztandera K., Gorzkiewicz M., Klajnert-Maculewicz B. Gold Nanoparticles in Cancer Treatment. Mol. Pharm. 2019. 16(1): 1. DOI: https://doi.org/10.1021/acs.molpharmaceut.8b00810
  2. Grumezescu A.M. (ed.) Nanobiomaterials in Cancer Therapy. Applications of nanobiomaterials. Vol. 7. Elsevier, 2016. DOI: https://doi.org/10.1016/C2015-0-00383-5
  3. Shi J., Kantoff P.W., Wooster R., Farokhzad O.C. Cancer nanomedicine: progress, challenges and opportunities. Nat. Rev. Cancer. 2017. 17(1): 20. DOI: https://doi.org/10.1038/nrc.2016.108
  4. Shmarakov I.O., Mukha Iu.P., Karavan V.V., Chunikhin O.Yu., Marchenko M.M., Smirnova N.P., Eremenko A.M. Tryptophan assisted synthesis reduces bimetallic gold/silver nanoparticle cytotoxicity and improves biological activity. Nanobiomedicine. 2014. 1: 1. DOI: https://doi.org/10.5772/59684
  5. Mukha Iu., Vityuk N., Severynovska O., Eremenko A., Smirnova N. The pH-dependent structure and properties of Au and Ag nanoparticles produced by tryptophan reduction. Nanoscale Res. Let. 2016. 11: 101. DOI: https://doi.org/10.1186/s11671-016-1318-8
  6. Vityuk N., Mukha Iu., Grodzyuk G., Eremenko A. Bimetallic gold/silver alloy nanoparticles prepared in the presence of tryptophan. Poverkhnia (Surface). 2016. 8: 118.
  7. Mukha Iu., Vityuk N., Grodzyuk G., Shcherbakov S., Lyberopoulou A., Efstathopoulos E.P., Gazouli M. Anticancer effect of Ag, Au, and Ag/Au bimetallic nanoparticles prepared in the presence of tryptophan. J. Nanosci. Nanotechnol. 2017. 17(12): 8987. DOI: https://doi.org/10.1166/jnn.2017.14106
  8. Katifelis H., Lyberopoulou A., Mukha Iu., Vityuk N., Grodzyuk G., Theodoropoulos G.E., Efstathopoulos E.P., Gazouli M. Ag/Au bimetallic nanoparticles induce apoptosis in human cancer cell lines via P53, CASPASE-3 and BAX/BCL-2 pathways. Artificial Cells, Nanomed. Biotechnol. 2018. 46(sup3): 389. DOI: https://doi.org/10.1080/21691401.2018.1495645
  9. Shmarakov I., Mukha Iu., Vityuk N., Borschovetska V., Zhyshchynska N., Grodzyuk G., Eremenko A. Antitumor Activity of Alloy and Core-Shell-Type Bimetallic AgAu Nanoparticles. Nanoscale Res. Let. 2017. 12(1): 333. DOI: https://doi.org/10.1186/s11671-017-2112-y  
  10. Mukha Iu., Vityuk N., Khodko A., Kachalova N., Fedyshyn O., Malysheva M., Eremenko A. Photo- and temperature-dependent formation of tryptophan/silver nanoparticles. Res. Chem. Interm. 2019. 45(8): 4053. DOI: https://doi.org/10.1007/s11164-019-03890-5
  11. Mukha I., Khodko A., Vityuk N., Severynovska O., Pivovarenko V., Kachalova N., Smirnova N., Eremenko A. Light-driven formation of gold/tryptophan nanoparticles. Appl. Nanosci. 2019. DOI: https://doi.org/10.1007/s13204-019-01171-6
  12. Khodko A., Kachalova N.; Scherbakov S., Eremenko A., Mukha Iu. Effects of photochromic furan-based diarylethenes on gold nanoparticles aggregation. Nanoscale Res. Let. 2017. 12(1): 271. DOI: https://doi.org/10.1186/s11671-017-2044-6  
  13. Pylypchuk Ie.V., Mukha Iu.P., Vityuk N.V., Szczepanowicz K., Storozhuk L.P., Eremenko A.M., Warszyński P., Gorbyk P.P. Tryptophan-Stabilized Plasmonic Fe3O4/Ag Nanoparticles. In: Fesenko O., Yatsenko L. (eds) Nanophotonics, Nanooptics, Nanobiotechnology, and Their Applications. Proc. NANO 2018. Vol. 222. Springer Cham., 2019. P. 417–430. DOI: https://doi.org/10.1007/978-3-030-17755-3_28
  14. Lyberopoulou A., Grammaticaki St., Mukha Iu., Vityuk N., Pylypchuk Ie., Storozhuk L., Kouloulias V., Gazouli Μ. In vitro effect of hyperthermic Αg and Au Fe3O4 nanoparticles in cancer cell cultures. Beilstein Arch. 2019, 2019101 (One-Day-Symposium on Nanotechnology in Health Science, 21, January, 2018, Athens, Greece). DOI: https://doi.org/10.3762/bxiv.2019.101.v1