Home /Archive /2018 /2018 №3 /2018_3_4

Visn. Nac. Akad. Nauk Ukr. 2018. (3): 19-32
https://doi.org/10.15407/visn2018.03.019

 

R.S. Stoika
Institute of Cell Biology of the National Academy of Sciences of Ukraine Lviv
https://orcid.org/0000-0001-5719-2187

PERSPECTIVES OF DEVELOPMENT OF THE NANOCARRIERS FOR DELIVERY OF GENETIC MATERIALS INTO CELLS
According to the materials of scientific report at the meeting of the Presidium of NAS of Ukraine, January 31, 2018

The most important results of the long-term experimental investigations conducted by scientists of the Institute of Cell Biology of NAS of Ukraine in collaboration with other institutions of the Academy and universities and addressed on the development and characteristics of the nanoscale carriers for efficient delivery of nucleic acids into cells of living organisms are presented in the review. Advantages and drawbacks of the viral (biological) and non-viral (chemical and physical) methods of gene delivery have been described. The first native non-viral gene delivery systems based on the comb-like and linear co-polymers with the polyampholytic properties have been synthesized at Lviv National Polytechnic University. Their ability to deliver genetic materials into cells of organisms of various taxonomic types (bacteria, yeast, and mammalian) was demonstrated at the Institute of Cell Biology of NAS of Ukraine. In collaboration with scientists of the Institute of Food Biotechnology and Genomics of NAS of Ukraine, first native polymeric nanocarriers for gene delivery into plant cells were also developed. The physico-chemical characteristics and biological effects of these gene delivery systems were compared with those of the polyethylenimine that is a widely used polymeric carrier of the nucleic acids. The biocompatibility of the developed systems, as well as the lack of their mutagenic activity, were demonstrated. Due to availability of specific chemical groups in the synthesized polymeric nanocarriers, it is possible to perform an addressed delivery of different biomolecules, particularly the DNA, that is impossible when using the traditional liposomal carriers of DNA. This can provide the carriers with ability to conduct addressed delivery of the biologically active molecules to target tissues with a simultaneous decreasing of their general toxicity and prolonging their therapeutic action in the treated organism. Thus, the availability of native nanocarriers of genetic materials that can be bought in the country should enhance gene engineering and gene therapy investigations in Ukraine.
Keywords: delivery of nucleic acids to cells, nanocarriers, multifunctional polymers.

Language of article: ukrainian

REFERENCES

  1. Pask A.J., Behringer R.R., Renfree M.B. Resurrection of DNA function in vivo from an extinct genome. PLoS One. 2008. 3(5): e2240. https://doi.org/10.1371/journal.pone.0002240
  2. Stoika R.S. (ed.). Multifunctional nanomaterials for biology and medicine: molecular design, synthesis and application. (Kyiv: Naukova Dumka, 2017).
  3. Lewin B. Genes VII. (Oxford University Press, New York, 2000). P. 533–539.
  4. Tanasienko I.V., Yemets A.I., Finiuk N.S., Stoika R.S., Blume Ya.B. DMAEM-based cationic polymers as novel carriers for DNA delivery into cells. Cell Biol. Int. 2014. 39(3): 243. https://doi.org/10.1002/cbin.10381
  5. Ficen S.Z., Guler Z., Mitina N., Finiuk N., Stoika R., Zaichenko A., Ceylan S.E. Biophysical study of novel oligoelectrolyte based non-viral gene delivery systems to mammalian cells. J. Gene Med. 2013. 15(5): 193. https://doi.org/10.1002/jgm.2710
  6. Finiuk N.S., Vitak T.Y., Mitina N.Y., Filyak Y.Z., Zaichenko O.S., Stoika R.S. Polyplex formation by novel surface active comb-like polyamfolytes and plasmid DNA. Biotechnologia Acta. 2012. 5(6): 66.
  7. Zimmer M. GFP: from jellyfish to the Nobel prize and beyond. Chem Soc Rev. 2009. 38(10): 2823. https://doi.org/10.1039/b904023d
  8. Finiuk N.S., Filyak Y.Z., Zaichenko O.S., Mitina N.Y., Stoika R.S. Using of the new nanosized polymer carrier for genetic transformation of bacterial cells. In: Microbial Biotechnology: Activities and Future (daRostim 2012): Proc. VIII Int. Conf. (Kyiv, 2012). P. 329–330.
  9. Filyak Y., Finiuk N., Mitina N., Bilyk O., Titorenko V., Hrydzhuk O., Zaichenko A., Stoika R. A novel method for genetic transformation of yeast cells using oligoelectrolyte polymeric nanoscale carriers. Biotechniques. 2013. 54(1): 35. https://doi.org/10.2144/000113980
  10. Filyak Y., Finiuk N., Mitina N., Zaichenko A., Stoika R. Application of novel polymeric carrier of plasmid DNA for transformation of yeast cells. In: Genetic transformation systems in Fungi. van den Berg, M.A., Maruthachalam K. (eds.). Vol. 1. (Springer, 2015). P. 201–207. https://doi.org/10.1007/978-3-319-10142-2_20
  11. Finiuk N.S., Chaplya A.Y., Mitina N.Y., Boiko N.M., Lobachevska O.V., Miahkota О.S., Yemets A.I., Blume Ya.B., Zaichenko O.S., Stoika R.S. Genetic transformation of the moss Ceratodon purpureus by novel polyсationic carriers of DNA. Cytology and Genetics.2014. 48(6): 345. https://doi.org/10.3103/S0095452714060048
  12. Finiuk N., Buziashvili A., Burlaka O., Zaichenko A., Mitina N., Miagkota O., Lobachevska O., Stoika R., Blume Ya., Yemets A. Investigation of novel oligoelectrolyte polymer carriers for their capacity of DNA delivery into plant cells. Plant Cell Tiss. Organ Cult. 2017. 131(1): 27. http://dx.doi.org/10.1007/s11240-017-1259-7
  13. Barry M.E., Pinto-Gonzalez D., Orson F.M., McKenzie G.J., Petry G.R., Barry M.A. Role of endogenous endonucleases and tissue site in transfection and CpG-mediated immune activation after naked DNA injection. Hum. Gene Therapy. 1999. 10(15): 2461. https://doi.org/10.1089/10430349950016816
  14. Lungwitz U., Breunig M., Blunk T., Göpferich A. Polyethylenimine-based non-viral gene delivery systems. Eur. J. Pharm. Biopharm. 2005. 60(2): 247. https://doi.org/10.1016/j.ejpb.2004.11.011
  15. Rudolph C., Ortiz A., Schillinger U., Jauernig J., Plank C., Rosenecker J. Methodological optimization of polyethylenimine (PEI)-based gene delivery to the lungs of mice via aerosol application. J. Gene Med. 2005. 7(1): 59. https://doi.org/10.1002/jgm.646
  16. Wang Y., Chen P., Shen J. The development and characterization of a glutathione-sensitive cross-linked polyethylenimine gene vector. Biomaterials. 2006. 27(30): 5292. https://doi.org/10.1016/j.biomaterials.2006.05.049
  17. Liu G., Swierczewska M., Lee S., Chen X. Functional nanoparticles for molecular imaging guided gene delivery. Nano Today. 2010. 5(6): 524. https://doi.org/10.1016/j.nantod.2010.10.005
  18. Sun X., Zhang N. Cationic polymer optimization for efficient gene delivery. Mini Rev. Med. Chem. 2010. 10(2): 108. http://dx.doi.org/10.2174/138955710791185109
  19. Marvaniya H.M., Parikh P.K., Patel V.R., Modi K.N., Sen D.J. Dendrimer nanocarriers as versatile vectors in gene delivery. J. Chem. Pharm. Res. 2010. 2(3): 97. http://www.jocpr.com/articles/dendrimer-nanocarriers-as-versatile-vectors-in-genedelivery.pdf
  20. Strand S.P., Lelu S., Reitan N.K., de Lange Davies C., Artursson P., Varum K.M. Molecular design of chitosan gene delivery systems with an optimized balance between polyplex stability and polyplex unpacking. Biomaterials. 2010. 31(5): 975. https://doi.org/10.1016/j.biomaterials.2009.09.102
  21. Tros de Larduya C., Sun Y., Düzgüneş N. Gene delivery by lipoplexes and polyplexes. Eur. J. Pharm. Sci. 2010. 40(3): 159. http://dx.doi.org/10.1016/j.ejps.2010.03.019
  22. Hu J., Kovtun A., Tomaszewski A., Singer B.B., Seitz B., Epple M., Steuhl K.-P., Ergun S., Fuchsluger T.A. A new tool for the transfection of corneal endothelial cells: Calcium phosphate nanoparticles. Acta Biomaterialia. 2012. 8(3): 1156. http://dx.doi.org/10.1016/j.actbio.2011.09.013
  23. Ljubimova J.Y., Fujita M., Ljubimov A.V., Torchilin V.P., Black K.L., Holler E. Poly(malic acid) nanoconjugates containing various antibodies and oligonucleotides for multitargeting drug delivery. Nanomedicine (Lond.). 2008. 3(2): 247. http://dx.doi.org/10.2217/17435889.3.2.247
  24. Madaan K., Kumar S., Poonia N., Lather V., Pandita D. Dendrimers in drug delivery and targeting: Drug-dendrimer interactions and toxicity issues. J. Pharm. Bioallied Sci. 2014. 6(3): 139. http://dx.doi.org/10.4103/0975-7406.130965
  25. Yin F., Hu K., Chen Y., Yu M., Wang D., Wang Q., Yong K.T., Lu F., Liang Y., Li Z. SiRNA delivery with PEGylated graphene oxide nanosheets for combined photothermal and genetherapy for pancreatic cancer. Theranostics. 2017. 7(5): 1133. http://dx.doi.org/10.7150/thno.17841
  26. Kobylyns’ka L.I., Havryliuk D.Ia., Riabtseva A.O., Mitina N.Ie., Zaichenko O.S., Zimenkovskyі B.S., Stoika R.S. Study of rat blood serum biochemical indicators of cardiotoxic action of novel antitumor 4-thiazolidinone derivatives and doxorubicin in complexes with polyethylene glycol-containing polymeric carrier in the rat blood serum. Ukr. Biochem. J. 2014. 86 (6): 84. http://dx.doi.org/10.15407/ubj86.06.084
  27. Kobylinska L.I., Havrylyuk D.Y., Ryabtseva A.O., Mitina N.E., Zaichenko O.S., Lesyk R.B., Zimenkovsky B.S., Stoika R.S. Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier. Ukr. Biochem. J. 2015. 87(2): 122. http://dx.doi.org/10.15407/ubj87.02.122
  28. Kоbylinska L.I., Havrylyuk D.Ya., Mitina N.E., Zаichenko A.S., Lesyk R.B., Zіmenkovsky B.S., Stoika R.S. Biochemical indicators of nephrotoxicity in blood serum of rats treated with novel 4-thiazolidinone derivatives or their complexes with polyethyleneglycol-containing nanoscale polymeric carrier. Ukr. Biochem. J. 2016. 88(1): 51. https://doi.org/10.15407/ubj88.01.051
  29. Finiuk N.S., Filyak Y.Z., Boiko N.M., Mitina N.Y., Zaichenko O.S., Stoika R.S. Evaluation of cytotoxic and mutagenic action of novel surface active comb-like polyampholytes that are used for delivery of nucleic acids to target cells. Biol. Studii. 2013. 7(2): 15.
  30. Patent of Ukraine No. 51916. Filyak Y., Stoika R., Hrydzhuk O. et al. Method for introduction of nucleic acids in the yeast cells. 10.08.2010.